Characterization of a cell culture persistently infected with the DA strain of Theiler's murine encephalomyelitis virus.
نویسندگان
چکیده
We established a persistent infection in L 929 cells with the DA strain of Theiler's murine encephalomyelitis virus. Our studies showed that only a small number of cells in the cultures contained infectious virus or viral antigen. A role for interferon in the maintenance of persistence was suggested. Viral isolates from the cultures were not temperature sensitive, nor did they contain viral capsid polypeptide mutations or defective interfering particles. T1 oligonucleotide maps showed evidence of mutation in two of three isolates.
منابع مشابه
Infectious RNA isolated from the spinal cords of mice chronically infected with Theiler's murine encephalomyelitis virus.
The DA strain of Theiler's murine encephalomyelitis virus (TMEV) persistently infects cells of the spinal cord during the chronic phase of infection. Although in situ hybridization and reverse transcription-PCR have demonstrated the presence of viral RNA in the spinal cord, it has not been determined whether this RNA is infectious and, if so, how many PFU equivalents of virus the RNA can yield....
متن کاملEarly infection of the central nervous system by the GDVII and DA strains of Theiler's virus.
The DA strain of Theiler's virus, a murine picornavirus, causes a persistent infection of glial cells of the white matter of the spinal cord, associated with chronic inflammation and primary demyelination. The GDVII strain causes an acute fatal grey matter encephalomyelitis. We characterized the target cells of GDVII and DA viruses 4 days following intracerebral inoculation, and we compared the...
متن کاملTheiler's virus and Mengo virus induce cross-reactive cytotoxic T lymphocytes restricted to the same immunodominant VP2 epitope in C57BL/6 mice.
C57BL/6 mice develop a virus-specific cytotoxic T-lymphocyte (CTL) response after intraperitoneal inoculation with either the DA strain of Theiler's virus or Mengo virus, two members of the Cardiovirus genus. These CTLs contribute to viral clearance in the case of Theiler's virus but do not protect the mice from the fatal encephalomyelitis caused by Mengo virus. In this study we show that DA an...
متن کاملThe leader peptide of Theiler's murine encephalomyelitis virus is a zinc-binding protein.
The leader (L) peptide is located in the amino-terminal part of the polyprotein of members of the Cardiovirus (which includes Theiler's murine encephalomyelitis virus) and Aphthovirus genera of picornaviruses. Although the function of L is unknown, strain DA of Theiler's murine encephalomyelitis virus with a mutation of L produces a cell-specific restricted infection. We now report that the DA ...
متن کاملL* protein of the DA strain of Theiler's murine encephalomyelitis virus is important for virus growth in a murine macrophage-like cell line.
Strain GDVII and other members of the GDVII subgroup of Theiler's murine encephalomyelitis virus (TMEV) are highly virulent and cause acute polioencephalomyelitis in mice. Neither viral persistence nor demyelination is demonstrated in the few surviving mice. On the other hand, strain DA and other members of the TO subgroup of TMEV are less virulent and establish a persistent infection in the sp...
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عنوان ژورنال:
- Journal of virology
دوره 43 3 شماره
صفحات -
تاریخ انتشار 1982